OneStep® Ovulation (LH) Test Cassettes (20 mIU/mL)


OneStep® Ovulation (LH) Test Cassettes are FDA cleared and CE approved.  These units detect LH at 20 mIU/mL.

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OneStep® Ovulation (LH) Test Cassettes are FDA cleared and CE approved.  These units detect LH at 20 mIU/mL.

You may check that this product is trusted and used by the National Health Service (NHS) in the UK by clicking here.

The cassette-style test utilises a pipette (included) to accurately add the required amount of urine to the test well. There is less chance of false positives/negatives with this style of test (when compared to test strips), but, they do cost more.


  • LH sensitivity: 20 mIU/mL

20mIU/mL ovulation tests are the most sensitive tests we stock. If you would like details on which sensitivity is best for you, please read our article here:

  • FDA and CE approved
  • Used by the NHS in the United Kingdom

Directions for use:

  • Make sure you do not open the package until you are ready to begin the test.
  • You should test your urine at the same time every day to get consistent results.
  • You should not test with urine from early morning (it is better to test from 10am onwards).
  • Do not drink too much two hours prior to performing the test, as doing so could dilute the sample and not show when you have a LH surge.
  1. Collect the urine sample in a clear container.
  2. Open the pouch and remove the cassette and pipette. Place the cassette on a flat surface.
  3. Using the pipette, draw 0.4ml (or 4 drops) of the sample urine.
  4. Dispense the sample (4 drops) into the cassette well.
  5. Wait for the results to show. For this test, a positive result may show as soon as 40 seconds after the sample is dispensed into the cassette. However, to confirm a negative result, you should wait for the full 10 minutes. Do NOT read the results after 30 minutes.

How to read the results:

Positive for LH surge: If two colour bands are visible, and the test band is equal to or darker than the control band, you will probably ovulate within the next 24-48 hours. If you are trying to get pregnant, you should have intercourse frequently over the next 48 hours.

Negative for LH surge: Only one colour band appears on the control region, or the test band appears but is lighter than the control band. This means there is no LH surge. You should continue daily testing.

Failed test: The control line has not appeared after 5 minutes. You should repeat the test with a new unit.

ovulation cassette test and results

Additional information: Please read the product datasheet for more information on this test and how it is performed. You can do so by clicking here.